Hamada Nobuyuki, Iwahashi Jun, Suzuki Kentaro, Ogi Hidenao, Kashiwagi Takahito, Hara Koyu, Toyoda Michiko, Yamada Tatsuo, Toyoda Tetsuya
Department of Virology, Kurume University School of Medicine, Fukuoka, Japan.
Cell Mol Biol (Noisy-le-grand). 2002 Mar;48(2):163-72.
cDNA of mouse reticulon 3 (mRTN3) was cloned. The cloned cDNA is 1745 bp long and contains an open reading frame of 237 amino acids for a 30 kDa protein. The gene was mapped at band B of chromosome 19 by FISH. Two altematively spliced transcripts, 3.4 and 2.3 kb, of mRTN3 were found by Northem blot analysis. Both transcripts were expressed in many tissues and embryos and the highest expression of the 3.4 kb-transcript was observed in the brain, especially in neurons. The expression of 30 kDa-mRTN3 protein was also greatest in the brain. Both N and C-termini of mRTN3 faced the cytosol, indicating that they may recruit other proteins to the endoplasmic reticulum.
小鼠网织蛋白3（mRTN3）的互补DNA（cDNA）被克隆出来。克隆出的cDNA长度为1745碱基对，包含一个由237个氨基酸组成的开放阅读框，编码一个30千道尔顿的蛋白质。通过荧光原位杂交（FISH）将该基因定位在19号染色体的B带。通过Northern印迹分析发现了mRTN3的两种选择性剪接转录本，分别为3.4千碱基和2.3千碱基。这两种转录本在许多组织和胚胎中均有表达，其中3.4千碱基转录本在脑中表达最高，尤其是在神经元中。30千道尔顿的mRTN3蛋白在脑中的表达也最为显著。mRTN3的N端和C端均面向细胞质溶胶，这表明它们可能将其他蛋白质招募到内质网。
