Mukhopadhyay I, Nazir A, Mahmood K, Saxena D K, Das M, Khanna S K, Chowdhuri D K
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow, India.
Cell Biol Toxicol. 2002;18(1):1-11. doi: 10.1023/a:1014433711554.
The effect of argemone oil on hsp70 expression and tissue damage was investigated by studying beta-galactosidase activity, Western blotting and hybridization, and trypan blue staining in the larval tissues of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9. Different concentrations of argemone oil were mixed with food and third-instar larvae were allowed to feed on them for different time intervals (2, 4, 24, and 48 h). Argemone oil was found to induce hsp70 even in the lowest concentration of the adulterant while maximum tissue damage was observed in the higher two treatment groups. Malpighian tubules and midgut tissue reflected maximum damage as evidenced by both high beta-galactosidase activity and trypan blue staining in these tissues. A prior temperature shock treatment to the larvae was enough to protect the larvae from argemone oil-induced tissue damage as evidenced by little or no trypan blue staining. The present study suggests the cytotoxic potential of argemone oil and further strengthens the evidence for the use of hsp70 as a biomarker in risk assessment.
通过研究转基因黑腹果蝇（hsp70-lacZ）Bg9幼虫组织中的β-半乳糖苷酶活性、蛋白质免疫印迹法和杂交以及台盼蓝染色，研究了白屈菜红碱对hsp70表达和组织损伤的影响。将不同浓度的白屈菜红碱与食物混合，让三龄幼虫以其为食不同时间间隔（2、4、24和48小时）。发现即使在最低浓度的掺假物中，白屈菜红碱也能诱导hsp70表达，而在较高的两个处理组中观察到最大的组织损伤。马氏管和中肠组织表现出最大损伤，这些组织中较高的β-半乳糖苷酶活性和台盼蓝染色证明了这一点。对幼虫进行预先的温度休克处理足以保护幼虫免受白屈菜红碱诱导的组织损伤，这一点从很少或没有台盼蓝染色可以证明。本研究表明白屈菜红碱具有细胞毒性潜力，并进一步加强了将hsp70用作风险评估生物标志物的证据。
