Mukhopadhyay Indranil, Saxena D K, Bajpai V K, Chowdhuri D Kar
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow 226001, Uttar Pradesh, India.
J Biochem Mol Toxicol. 2003;17(4):223-34. doi: 10.1002/jbt.10082.
We explored the reproductive toxicity of argemone oil and its principal alkaloid fraction in transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9). The toxicity of argemone oil has been attributed to two of its physiologically active benzophenanthridine alkaloids, sanguinarine and dihydrosanguinarine. Freshly eclosed first instar larvae of transgenic Drosophila melanogaster were transferred to different concentrations of argemone oil and its alkaloid fraction contaminated food. Virgin flies that eclosed from the contaminated food were pair-mated to look into the effect on reproduction. The study was further extended by investigating hsp70 expression and tissue damage in larval gonads, genital discs, and reproductive organs of adult fly. Our results showed that argemone oil was more cytotoxic than its principal alkaloid fraction. Moreover, it was the male fly that was more affected compared to its opposite number. The accessory glands of male reproductive system of the fly, which did not express hsp70, exhibited severe damage as evidenced by Trypan blue staining. This prompted us to explore the ultrastructural morphology of the gland, which showed acute signs of necrosis in both the cell types as evident by necrotic nuclei, higher vacuolization, and disorganized endoplasmic reticulum, decrease in the number of Golgi vesicles and disorganized, loosely packed filamentous structures in the lumen of the accessory gland, at the higher concentrations of the adulterant. The study showed the reproductive toxicity of argemone oil and its alkaloid fraction in transgenic Drosophila melanogaster and further confirmed the cytoprotective role of hsp70.
我们研究了白屈菜红碱及其主要生物碱组分对转基因黑腹果蝇（hsp70-lacZ）Bg(9)的生殖毒性。白屈菜红碱的毒性归因于其两种具有生理活性的苯并菲啶生物碱——血根碱和二氢血根碱。将新羽化的转基因黑腹果蝇一龄幼虫转移至含有不同浓度白屈菜红碱及其生物碱组分的受污染食物中。从受污染食物中羽化出的未交配果蝇进行配对，以研究其对繁殖的影响。通过研究幼虫性腺、生殖盘以及成年果蝇生殖器官中的hsp70表达和组织损伤，进一步拓展了该研究。我们的结果表明，白屈菜红碱比其主要生物碱组分具有更强的细胞毒性。此外，雄性果蝇比雌性果蝇受到更大影响。果蝇雄性生殖系统的附腺未表达hsp70，经台盼蓝染色显示出严重损伤。这促使我们探究该腺体的超微结构形态，结果显示，在较高浓度掺假物作用下，两种细胞类型均出现急性坏死迹象，表现为细胞核坏死、空泡化增加、内质网紊乱、高尔基体囊泡数量减少以及附腺管腔内丝状结构排列紊乱且松散。该研究表明了白屈菜红碱及其生物碱组分对转基因黑腹果蝇的生殖毒性，并进一步证实了hsp70的细胞保护作用。
