Wittmann C
Biochemical Engineering Institute, Saarland University, Saarbruecken, Germany.
Adv Biochem Eng Biotechnol. 2002;74:39-64. doi: 10.1007/3-540-45736-4_3.
Detailed knowledge on carbon flux distributions is crucial for the understanding and targeted optimization of cellular systems. Analytical methods to identify the topology of metabolic networks and to quantify fluxes through its different pathways are therefore in the core of metabolic engineering. An elegant approach for metabolic flux analysis is provided by tracer experiments. In such studies tracer substrates with stable isotopes such as 13C are applied and the labeling pattern of metabolites is subsequently measured. Detailed flux distributions can be obtained by a combination of tracer experiments and stoichiometric balancing. In recent years, mass spectrometry (MS) has emerged as an interesting method for labeling measurements in metabolic flux analysis and provided valuable insights into the cellular metabolism. The present review provides an overview on current experimental and modeling tools for metabolic flux analysis by MS. The application of MS for flux analysis is illustrated by examples from the literature for various biological systems, including bacteria, fungi, tissue cultures and in vivo studies in humans.
深入了解碳通量分布对于理解细胞系统并进行有针对性的优化至关重要。因此，识别代谢网络拓扑结构并量化其不同途径通量的分析方法是代谢工程的核心。示踪实验为代谢通量分析提供了一种巧妙的方法。在这类研究中，会应用含有稳定同位素（如¹³C）的示踪底物，随后测量代谢物的标记模式。通过示踪实验和化学计量平衡相结合，可以获得详细的通量分布。近年来，质谱（MS）已成为代谢通量分析中用于标记测量的一种有趣方法，并为细胞代谢提供了有价值的见解。本综述概述了当前用于通过质谱进行代谢通量分析的实验和建模工具。通过文献中各种生物系统（包括细菌、真菌、组织培养和人体体内研究）的实例说明了质谱在通量分析中的应用。
