van der Heijden F L, James J
Z Mikrosk Anat Forsch. 1975;89(1):18-26.
In an investigation to determine whether the enlargement of cells and nuclei in the myometrium of the human uterus during pregnancy is related to the development of polyploidy or not, the following facts were established, mainly on the basis of cytophotometric analysis of nuclei isolated with a newly developed mechanical technique from a series of uteri in different states with regard to the reproductive process (juvenile, nulligravida, gravida, sectio parva, sectio caesarea). 1. Polyploid nuclei arise only during pregnancy. They can still be found for many years after the puerperium; their occurrence, however, remains a discrete phenomenon. 2. During pregnancy, a swelling of virtually all diploid nuclei is observed, which is present as early as 16 weeks after conception, but a significant increase of the nuclear projection area is found only in the Caesarean section group. This swelling, which (might occur under hormonal influence is possibly of a functional nature and is separate from the development of polyploidy as such. 3. By means of the tracing of double sex chromatin bodies in the nuclei, the occurrence of tetraploid nuclei in the myometrium could be demonstrated both in nuclear suspensions and in sections of intact uterine wall.
在一项旨在确定妊娠期间人类子宫肌层中细胞和细胞核增大是否与多倍体发育相关的研究中，主要基于用一种新开发的机械技术从一系列处于不同生殖状态（幼年、未孕、妊娠、剖宫产、顺产）的子宫中分离出的细胞核进行细胞光度分析，确立了以下事实。1. 多倍体核仅在妊娠期间出现。产后多年仍可发现它们；然而，它们的出现仍然是一种离散现象。2. 在妊娠期间，几乎所有二倍体核都出现肿胀，早在受孕后16周就已存在，但仅在剖宫产组中发现核投影面积有显著增加。这种肿胀（可能在激素影响下发生）可能具有功能性质，并且与多倍体本身的发育是分开的。3. 通过追踪细胞核中的双性染色质体，在核悬浮液和完整子宫壁切片中均能证明子宫肌层中四倍体核的存在。
