Ishchenko A M, Moskvicheva I V, Shapiro N I
Zh Mikrobiol Epidemiol Immunobiol. 1975 Sep;0(9):71-4.
A study was made of the hydrodynamic and biological properties of the flagella from the S. typhi 4446 cultures, isolated by the methods of differential centrifugation, in crude condition and following depolimerization and denaturing by the action of chemical and physical agents. Molecular parameters of the slow and rapid components of the flagella and subunits of the flagellin were compared. The greatest antigenic activity was possessed by the high molecular fraction of the flagella isolated in the 60% sucrose density gradient.
对通过差速离心法从伤寒沙门氏菌4446培养物中分离出的鞭毛在粗提状态下以及在化学和物理试剂作用下进行解聚和变性后的流体动力学和生物学特性进行了研究。比较了鞭毛的慢速和快速组分以及鞭毛蛋白亚基的分子参数。在60%蔗糖密度梯度中分离出的鞭毛高分子级分具有最大的抗原活性。