dos Santos L A, Carrodéguas R G, Rogero S O, Higa O Z, Boschi A O, de Arruda A C F
Universidade Estadual de Campinas, UNICAMP-FEM CEP, SP, Brazil.
Biomaterials. 2002 May;23(9):2035-42. doi: 10.1016/s0142-9612(01)00333-7.
Calcium phosphate-based bioceramics have revolutionized orthopedic and dental repair of damaged parts of the bone system. Among these materials, calcium phosphate-based cements, with hydraulic setting, stand out due to their biocompatibility and in situ hardening, which allow easy manipulation and adaptation to the shape and dimensions of bone defects. An investigation was made of the in vitro cytotoxic effect of calcium phosphate cement based on alpha-tricalcium phosphate, immersed for different lengths of time in simulated body fluid (SBF), based on the ISO-10993 "Biological Evaluation of Medical Devices" standard. The culture medium was Chinese hamster ovary (CHO) cells in contact with diluted cement extracts. The results revealed that the calcium phosphate cement used was cytotoxic and that the material's cytotoxicity decreased the longer the cement was immersed in SBF.
基于磷酸钙的生物陶瓷彻底改变了骨系统受损部位的骨科和牙科修复。在这些材料中，具有水硬性固化的磷酸钙基骨水泥因其生物相容性和原位硬化而脱颖而出，这使得它们易于操作并能适应骨缺损的形状和尺寸。基于ISO-10993“医疗器械生物学评价”标准，对基于α-磷酸三钙的磷酸钙骨水泥在模拟体液（SBF）中浸泡不同时长后的体外细胞毒性作用进行了研究。培养基为与稀释的骨水泥提取物接触的中国仓鼠卵巢（CHO）细胞。结果显示，所使用的磷酸钙骨水泥具有细胞毒性，且该材料的细胞毒性会随着骨水泥在SBF中浸泡时间的延长而降低。
