Shi Guoyue, Liu Min, Zhu Min, Zhou Tianshu, Chen Junshui, Jin Litong, Jin Ji-Ye
Department of Chemistry, East China Normal University, Shanghai.
Analyst. 2002 Mar;127(3):396-400. doi: 10.1039/b108462n.
A novel hypoxanthine (Hx) microsensor was constructed. In this work, Nafion xanthine oxidase (XOD) and Au colloid were immobilized onto the surface of a Pt microelectrode. The enzyme biosensor displayed a quick and sensitive response to Hx. Under physiological conditions, a low detection limit, with high selectivity and sensitivity for Hx determination were obtained. The oxidation current [investigated using current-time (I-t) plots] was linear with Hx concentration ranging from 2.0 x 10(-7) to 2.0 x 10(-5) mol L(-1) with a calculated detection limit of 1.0 x 10(-7) mol L(-1) (S/N of 3). The biosensor should be promising for in vivo measurement of Hx without interferences and fouling. The change of Hx concentration in cardiac myocytes stimulated by L-arginine (L-Arg) and acetylcholine (Ach) was also studied.
构建了一种新型次黄嘌呤（Hx）微传感器。在这项工作中，将Nafion黄嘌呤氧化酶（XOD）和金胶体固定在铂微电极表面。该酶生物传感器对Hx表现出快速且灵敏的响应。在生理条件下，获得了低检测限，对Hx测定具有高选择性和灵敏度。氧化电流[使用电流-时间（I-t）曲线进行研究]与Hx浓度在2.0×10⁻⁷至2.0×10⁻⁵ mol L⁻¹范围内呈线性关系，计算得出的检测限为1.0×10⁻⁷ mol L⁻¹（信噪比为3）。该生物传感器有望用于体内Hx的测量，且无干扰和污染。还研究了L-精氨酸（L-Arg）和乙酰胆碱（Ach）刺激下心肌细胞中Hx浓度的变化。
