El Harrad Loubna, Amine Aziz
Laboratoire Génie des Procédés et Environnement, Faculté de Sciences et Techniques, Hassan II University of Casablanca, B.P.146, Mohammedia, Morocco.
Laboratoire Génie des Procédés et Environnement, Faculté de Sciences et Techniques, Hassan II University of Casablanca, B.P.146, Mohammedia, Morocco.
Enzyme Microb Technol. 2016 Apr;85:57-63. doi: 10.1016/j.enzmictec.2016.01.006. Epub 2016 Jan 18.
A simple and sensitive amperometric biosensor was developed for the screening of potential xanthine oxidase inhibitors from medicinal plants. This biosensor was prepared by immobilization of xanthine oxidase on the surface of prussian blue modified screen-printed electrodes using nafion and glutaraldehyde. The developed biosensor showed a linear amperometric response at an applied potential of +0.05 V toward the detection of hypoxanthine from 5 μM to 45 μM with a detection limit of 0.4 μM (S/N=3) and its sensitivity was found to be 600 mA M(-1) cm(-2). In addition, the biosensor exhibited a good storage stability. The inhibition of xanthine oxidase by allopurinol was studied under the optimized conditions. The linear range of allopurinol concentration is obtained up to 2.5 μM with an estimated 50% of inhibitionI50=1.8 μM. The developed biosensor was successfully applied to the screening of xanthine oxidase inhibitors from 13 medicinal plants belonging to different families. Indeed, Moroccan people traditionally use these plants as infusion for the treatment of gout and its related symptoms. For this purpose, water extracts obtained from the infusion of these plants were used for the experiments. In this work, 13 extracts were assayed and several of them demonstrated xanthine oxidase inhibitory effect, with an inhibition greater than 50% compared to spectrophotometry measurements that only few extracts showed an inhibition greater than 50%.
开发了一种简单灵敏的安培型生物传感器，用于从药用植物中筛选潜在的黄嘌呤氧化酶抑制剂。该生物传感器是通过使用萘酚和戊二醛将黄嘌呤氧化酶固定在普鲁士蓝修饰的丝网印刷电极表面制备而成。所开发的生物传感器在 +0.05 V 的施加电位下对 5 μM 至 45 μM 的次黄嘌呤检测呈现线性安培响应，检测限为 0.4 μM（S/N = 3），其灵敏度为 600 mA M(-1) cm(-2)。此外，该生物传感器表现出良好的储存稳定性。在优化条件下研究了别嘌呤醇对黄嘌呤氧化酶的抑制作用。别嘌呤醇浓度的线性范围可达 2.5 μM，估计 50%抑制浓度 I50 = 1.8 μM。所开发的生物传感器成功应用于从 13 种不同科的药用植物中筛选黄嘌呤氧化酶抑制剂。实际上，摩洛哥人传统上使用这些植物的浸液来治疗痛风及其相关症状。为此，将这些植物浸液获得的水提取物用于实验。在这项工作中，对 13 种提取物进行了测定，其中几种表现出黄嘌呤氧化酶抑制作用，与分光光度法测量相比，抑制率大于 50%，而分光光度法测量只有少数提取物显示抑制率大于 50%。
