• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines.

作者信息

Lefeber Dirk J, Gutiérrez Gallego Ricardo, Grün Christian H, Proietti Daniela, D'Ascenzi Sandro, Costantino Paolo, Kamerling Johannis P, Vliegenthart Johannes F G

机构信息

Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, PO Box 80.075, NL-3508 TB, The Netherlands.

出版信息

Carbohydr Res. 2002 Apr 30;337(9):819-25. doi: 10.1016/s0008-6215(02)00059-9.

DOI:10.1016/s0008-6215(02)00059-9
PMID:11996835
Abstract

A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [-->3)-beta-D-GlcpA-(1-->4)-beta-D-Glcp-(1-->] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by 1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.

摘要

相似文献

1
Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines.
Carbohydr Res. 2002 Apr 30;337(9):819-25. doi: 10.1016/s0008-6215(02)00059-9.
2
Development of a high-performance anion-exchange chromatography with pulsed-amperometric detection based quantification assay for pneumococcal polysaccharides and conjugates.基于脉冲安培检测的肺炎球菌多糖及结合物高效阴离子交换色谱定量分析方法的建立。
Vaccine. 2002 Jun 7;20(19-20):2474-84. doi: 10.1016/s0264-410x(02)00183-4.
3
Quantitative determination of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharides by use of high-performance anion-exchange chromatography with pulsed amperometric detection.采用高效阴离子交换色谱-脉冲安培检测法对肺炎链球菌荚膜多糖中的C-多糖进行定量测定。
Vaccine. 2001 Apr 6;19(20-22):2987-94. doi: 10.1016/s0264-410x(00)00535-1.
4
The cell wall polysaccharide of Streptococcus gordonii 38: structure and immunochemical comparison with the receptor polysaccharides of Streptococcus oralis 34 and Streptococcus mitis J22.戈登链球菌38的细胞壁多糖：与口腔链球菌34和缓症链球菌J22的受体多糖的结构及免疫化学比较
Glycobiology. 1994 Apr;4(2):183-92. doi: 10.1093/glycob/4.2.183.
5
Synthesis of oligosaccharides related to the repeating unit of the capsular polysaccharide from Streptococcus pneumoniae type 37.与肺炎链球菌37型荚膜多糖重复单元相关的寡糖的合成
Carbohydr Res. 2005 Jan 17;340(1):7-13. doi: 10.1016/j.carres.2004.11.004.
6
Isolation and structural characterization of beta-D-glucosyluronic acid and 4-O-methyl beta-D-glucosyluronic acid-containing oligosaccharides from the cell-wall pectic polysaccharide, rhamnogalacturonan I.从细胞壁果胶多糖鼠李半乳糖醛酸聚糖I中分离并鉴定含β-D-葡萄糖醛酸和4-O-甲基-β-D-葡萄糖醛酸的寡糖
Carbohydr Res. 1994 Jan 15;252:235-43. doi: 10.1016/0008-6215(94)90018-3.
7
Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines against Neisseria meningitidis groups Y and W135.用于制备抗Y群和W135群脑膜炎奈瑟菌结合疫苗的细菌荚膜寡糖的大小测定
Vaccine. 2005 Mar 14;23(16):1887-99. doi: 10.1016/j.vaccine.2004.10.021.
8
Synthesis of four spacer-containing 'tetrasaccharides' that represent four possible repeating units of the capsular polysaccharide of Streptococcus pneumoniae type 6B.合成四种含间隔基的“四糖”，它们代表肺炎链球菌6B型荚膜多糖的四种可能重复单元。
Carbohydr Res. 1998 Jan;306(1-2):111-25. doi: 10.1016/s0008-6215(97)10013-1.
9
Chemo-enzymatic synthesis of tetra-, penta-, and hexasaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 14.14型肺炎链球菌荚膜多糖四糖、五糖和六糖片段的化学酶法合成
Carbohydr Res. 2003 Nov 14;338(23):2629-51. doi: 10.1016/s0008-6215(03)00292-1.
10
Development of a new method for the quantitative analysis of the extracellular polysaccharide of Neisseria meningitidis serogroup A by use of high-performance anion-exchange chromatography with pulsed-amperometric detection.开发一种利用高效阴离子交换色谱-脉冲安培检测法定量分析A群脑膜炎奈瑟菌细胞外多糖的新方法。
Vaccine. 2001 Feb 28;19(15-16):1989-97. doi: 10.1016/s0264-410x(00)00427-8.

引用本文的文献

1
Impact and Control of Sugar Size in Glycoconjugate Vaccines.糖大小对糖缀合物疫苗的影响和控制。
Molecules. 2022 Sep 29;27(19):6432. doi: 10.3390/molecules27196432.
2
Glycoconjugate synthesis using chemoselective ligation.糖缀合物的化学选择性连接合成。
Org Biomol Chem. 2019 Mar 6;17(10):2646-2650. doi: 10.1039/c9ob00270g.
3
Novel polysaccharide-protein conjugates provide an immunogenic 13-valent pneumococcal conjugate vaccine for .新型多糖-蛋白质结合物提供了一种用于……的具有免疫原性的13价肺炎球菌结合疫苗。 （注：原文句子不完整，for后面缺少内容）
Synth Syst Biotechnol. 2017 Jan 20;2(1):49-58. doi: 10.1016/j.synbio.2016.12.002. eCollection 2017 Mar.
4
Profiling pneumococcal type 3-derived oligosaccharides by high resolution liquid chromatography-tandem mass spectrometry.通过高分辨率液相色谱-串联质谱法分析3型肺炎球菌衍生的寡糖。
J Chromatogr A. 2015 Jun 5;1397:43-51. doi: 10.1016/j.chroma.2015.04.009. Epub 2015 Apr 11.