编码在克氏锥虫无鞭毛体中表达的核糖核蛋白抗原的cDNA克隆的鉴定。
Characterization of cDNA clones encoding ribonucleoprotein antigens expressed in Trypanosoma cruzi amastigotes.
作者信息
DaRocha Wanderson D, Bartholomeu Daniella C, Macêdo Camila D S, Horta M Fátima, Cunha-Neto Edécio, Donelson John E, Teixeira Santuza M R
机构信息
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
出版信息
Parasitol Res. 2002 Apr;88(4):292-300. doi: 10.1007/s00436-001-0540-0.
Sera from patients with Chagas' disease were used to screen a Trypanosoma cruzi amastigote cDNA library. Characterization of 50 positive clones showed that 21 (42%) encode previously identified T. cruzi ribosomal and flagellar proteins, heat-shock proteins or proteins with repetitive motifs. Twenty-nine clones (58%) correspond to nine genes not previously described in T. cruzi. Three cDNAs, encoding novel repetitive antigens with homology to ribosomal proteins and to other RNA binding proteins, were further characterized. Patient humoral responses against the recombinant proteins encoded by these cDNAs were evaluated in anticipation that they may constitute potential new targets for serodiagnostic assays.
来自恰加斯病患者的血清被用于筛选克氏锥虫无鞭毛体cDNA文库。对50个阳性克隆的特征分析表明，21个（42%）编码先前鉴定出的克氏锥虫核糖体蛋白、鞭毛蛋白、热休克蛋白或具有重复基序的蛋白。29个克隆（58%）对应于9个先前未在克氏锥虫中描述的基因。对三个编码与核糖体蛋白和其他RNA结合蛋白具有同源性的新型重复抗原的cDNA进行了进一步表征。预期这些cDNA编码的重组蛋白可能构成血清诊断检测的潜在新靶点，因此对患者针对这些重组蛋白的体液免疫反应进行了评估。
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