多种含有串联重复氨基酸序列基序的克氏锥虫抗原。

Multiple Trypanosoma cruzi antigens containing tandemly repeated amino acid sequence motifs.

作者信息

Ibañez C F, Affranchino J L, Macina R A, Reyes M B, Leguizamon S, Camargo M E, Aslund L, Pettersson U, Frasch A C

机构信息

Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina.

出版信息

Mol Biochem Parasitol. 1988 Jul;30(1):27-33. doi: 10.1016/0166-6851(88)90129-6.

DOI:10.1016/0166-6851(88)90129-6

PMID:3135494

Abstract

Chromosomal DNA from Trypanosoma cruzi, the agent of the American trypanosomiasis (Chagas' disease), was used for construction of a DNA library, employing the expression vector lambda gt11. Nine clones encoding different parasite antigens were isolated from this library by screening with an antiserum from a Chagasic patient. Nucleotide sequence analysis showed that seven out of the nine isolated clones code for antigens which contain tandemly repeated amino acid sequence motifs. Each of the seven antigens contains a unique repeat, ranging in length between 5 and 68 amino acids. The length of the repeats is highly conserved within each clone. Fusion proteins, expressed from two of the clones, reacted with a large proportion of sera collected from Chagasic patients in Argentina, Brazil and Chile. These clones appear thus to encode antigens which are shared between different strains of T. cruzi. Immunofluorescence experiments with live parasites showed that three of the antigens were detectable on the surface of trypanosomes.

摘要

来自美洲锥虫病（恰加斯病）病原体克氏锥虫的染色体DNA，被用于构建一个DNA文库，采用的表达载体是λgt11。通过用一名恰加斯病患者的抗血清进行筛选，从该文库中分离出九个编码不同寄生虫抗原的克隆。核苷酸序列分析表明，九个分离克隆中的七个编码的抗原含有串联重复的氨基酸序列基序。这七种抗原中的每一种都包含一个独特的重复序列，长度在5到68个氨基酸之间。每个克隆内重复序列的长度高度保守。从其中两个克隆表达的融合蛋白，与从阿根廷、巴西和智利的恰加斯病患者收集的大部分血清发生反应。因此，这些克隆似乎编码克氏锥虫不同菌株之间共有的抗原。对活寄生虫进行的免疫荧光实验表明，其中三种抗原在锥虫表面可检测到。