Bachelet Maria, Pinot Françoise, Polla Rachel I, François Dominique, Richard Marie-Jeanne, Vayssier-Taussat Muriel, Polla Barbara S
Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, Paris, France.
Free Radic Res. 2002 Jan;36(1):99-106. doi: 10.1080/10715760210159.
The effects of cadmium, an environmental toxin present in tobacco smoke, were studied in vitro in human monocytes and compared to those of tobacco smoke. Overexpression of the 72kDa heat shock/stress protein Hsp70 and cell death occurred with a similar time-course and to a similar extent in human monocytes exposed to either cadmium or tobacco smoke. Cadmium and tobacco smoke-mediated toxicity were associated with a decrease in the cellular content of glutathione and ATP and the glutathione precursor N-acetyl-L-cysteine prevented both cadmium and tobacco smoke-mediated toxicity. Furthermore, tobacco smoke-mediated toxicity was prevented by pretreatment with the cadmium chelator resin Chelex-100, supporting the conclusion that cadmium plays a major role in tobacco smoke-mediated toxicity.
研究了烟草烟雾中存在的环境毒素镉对人单核细胞的体外影响，并与烟草烟雾的影响进行了比较。在暴露于镉或烟草烟雾的人单核细胞中，72kDa热休克/应激蛋白Hsp70的过表达和细胞死亡以相似的时间进程和相似的程度发生。镉和烟草烟雾介导的毒性与谷胱甘肽和ATP的细胞含量降低有关，谷胱甘肽前体N-乙酰-L-半胱氨酸可预防镉和烟草烟雾介导的毒性。此外，用镉螯合剂树脂Chelex-100预处理可预防烟草烟雾介导的毒性，支持镉在烟草烟雾介导的毒性中起主要作用的结论。
