Kaiho M, Nakamura T, Kumegawa M
Anat Rec. 1975 Nov;183(3):405-19. doi: 10.1002/ar.1091830305.
The synthesis of zymogen-like secretory granules in convoluted tubules of mouse submandibular gland (SMG) was investigated by histometry, light microscopy and electron microscopy. In normal males secretory granules in the SMG increased greatly from 25 days after birth and reached a maximum level 50 days after birth. Castration of adult male mice markedly decreased the level, but it was completely restored by testosterone administration. A parallel was found between change in the granule level and the amount of rough endoplasmic reticulum (RER) in the convoluted tubular cells during development or after various treatments. Development of the Golgi apparatus was also observed in the cells when the granules increase. Both the increase in the granules and in the RER induced by testosterone were prevented by actinomycin D or puromycin. These results indicate that the granule contents are synthesized on the RER under the control of testosterone, and then condensed in the Golgi apparatus.
通过组织计量学、光学显微镜和电子显微镜研究了小鼠下颌下腺（SMG）曲管中类酶原分泌颗粒的合成。在正常雄性小鼠中，SMG中的分泌颗粒从出生后25天开始大幅增加，并在出生后50天达到最高水平。成年雄性小鼠去势后颗粒水平显著降低，但通过给予睾酮可使其完全恢复。在发育过程中或经过各种处理后，颗粒水平的变化与曲管细胞中粗面内质网（RER）的量之间发现了平行关系。当颗粒增加时，细胞中也观察到了高尔基体的发育。放线菌素D或嘌呤霉素可阻止睾酮诱导的颗粒和RER的增加。这些结果表明，颗粒内容物在睾酮的控制下在RER上合成，然后在高尔基体中浓缩。
