Read Timothy D, Salzberg Steven L, Pop Mihai, Shumway Martin, Umayam Lowell, Jiang Lingxia, Holtzapple Erik, Busch Joseph D, Smith Kimothy L, Schupp James M, Solomon Daniel, Keim Paul, Fraser Claire M
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA., Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.
Science. 2002 Jun 14;296(5575):2028-33. doi: 10.1126/science.1071837. Epub 2002 May 9.
Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.
将从近期生物恐怖主义炭疽袭击受害者身上分离出的炭疽芽孢杆菌全基因组序列与参考序列进行比较，发现了60个新标记，包括单核苷酸多态性（SNP）、插入或缺失序列以及串联重复序列。基因组比较在两条已测序的炭疽芽孢杆菌染色体之间检测到4个高质量SNP，在参考基因组的不同制备物之间检测到7个差异。这些标记已在一组炭疽分离株上进行了测试，发现可将这些样本分为不同的家族。这些结果表明，基于基因组的微生物病原体分析将为传染病暴发调查提供一个强大的新工具。
