控制性卵巢过度刺激后不同大小卵泡来源的黄素化颗粒细胞中胰岛素样生长因子（IGF）、IGF受体及IGF结合蛋白信使核糖核酸的表达
Expression of insulin-like growth factor (IGF), IGF receptor, and IGF-binding protein messenger ribonucleic acids in luteinized granulosa cells from different size follicles after controlled ovarian hyperstimulation.
作者信息
Chang Shiuh Y, Tsai Meng-Yin, Huang Fu-Jen, Kung Fu-Tsai
机构信息
Medical School, Chang-Gung University, Taoyuan, Taiwan.
出版信息
J Assist Reprod Genet. 2002 Mar;19(3):121-6. doi: 10.1023/a:1014732704218.
PURPOSE
This study was conducted to determine the gene expression of insulin-like growth factors (IGFs), their receptors, and binding proteins (IGFBPs) in human luteinized granulosa cells collected during ovum retrieval.
METHODS
cDNA probes were used to analyze the gene expression of IGF-I, IGF-II, IGF type 1 receptor (IGFR-1), IGF type 2 receptor (IGFR-2), and IGFBP-1 to -6 in luteinized granulosa cells.
RESULTS
The levels of IGFBP-5 transcripts of the luteinized granulosa cells increased with increasing follicular fluid (FF) volume when follicles were grouped into 3 volume catagories of < or = 1.5 mL: > 1.5 mL but < or = 4.5 mL; and > 4.5 mL (0.28 +/- 0.64 vs. 0.41 +/- 0.76 vs. 0.46 +/- 0.92, p = 0.037).
CONCLUSIONS
The increased levels of IGFBP-5 transcripts in luteinized granulosa cells found with increased follicular fluid volume after COH may favor a shift toward diminished action of IGFs in larger follicles immediately prior to ovulation.
目的
本研究旨在确定在取卵过程中收集的人黄素化颗粒细胞中胰岛素样生长因子（IGFs）、其受体及结合蛋白（IGFBPs）的基因表达情况。
方法
使用cDNA探针分析黄素化颗粒细胞中IGF-I、IGF-II、1型IGF受体（IGFR-1）、2型IGF受体（IGFR-2）以及IGFBP-1至-6的基因表达。
结果
当卵泡被分为<或=1.5 mL、>1.5 mL但<或=4.5 mL、>4.5 mL这3种体积类别时，黄素化颗粒细胞中IGFBP-5转录本水平随卵泡液（FF）体积增加而升高（0.28±0.64对0.41±0.76对0.46±0.92，p = 0.037）。
结论
在控制性卵巢刺激（COH）后，随着卵泡液体积增加，黄素化颗粒细胞中IGFBP-5转录本水平升高，这可能有利于在排卵前较大卵泡中向IGFs作用减弱的方向转变。
Zotero 插件