Dynamics of intracellular calcium induced by lactate and glucose in rat pachytene spermatocytes and round spermatids.

Glycolytic metabolism in meiotic and post-meiotic spermatogenic cells shows differentiation-related changes. The developmental and physiological significance of these metabolic changes is not known. The aim of the present study was to test the hypothesis that glucose and lactate metabolism can modulate intracellular calcium Ca2+ in spermatogenic cells in an opposing and dynamic manner. Fluorescent probes were used to measure Ca2+ and pH(i), and HPLC was used to measure intracellular adenine nucleotides and mitochondrial sensing of ATP turnover. Ca2+ in pachytene spermatocytes and round spermatids was modulated by changes in lactate and glucose concentrations in the media. The kinetics and magnitude of the Ca2+ changes induced by lactate and glucose were different in meiotic and post-meiotic spermatogenic cells. The presence of glucose in the medium induced a decrease in pH(i) in spermatogenic cells. This glucose-induced pH(i) decrease occurred later than the changes in Ca2+, which were also observed when the pH(i) decrease was inhibited, indicating that the glucose-induced Ca2+ increase was not a consequence of pH(i) changes. Hexose phosphorylation in glycolysis was part of the mechanism by which glucose metabolism induced a Ca2+ increase in spermatogenic cells. The sensitivity of Ca2+ to carbohydrate metabolism was higher in round spermatids than in pachytene spermatocytes. Thus, differentiation-related changes in carbohydrate metabolism in spermatogenic cells determine a dynamic and differential modulation of their Ca2+ by glucose and lactate, two substrates secreted by the Sertoli cells.