Zhou Dong-Ming, Yi Xin-Yuan, Zeng Xian-Fang, Wang Min, Cai Cai, Wang Qing-Lin, Zhang Shun-Ke, McReynolds Larry
Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Mar;34(2):143-8.
In order to explore the molecular mechanism of high immune protection against schistosomes infection in animals infected with Trichinella spiralis, and to provide several cross-protective antigen genes for developing anti-schistosomiasis vaccine, a Schistosoma japonicum adult worm cDNA library was immunoscreened using sera taken from mice infected with Trichinella spiralis. Nine positive clones were obtained after 3 rounds of immunoscreening. Among them, Sj-Ts4 represents a novel gene of S. japonicum, which coding for a novel protein of 210 amino acids. The protein has a deduced molecular mass of 23 kD and isoelectric point of 7.72. Sj-Ts4 was expressed as a glutathione-S-transferase (GST) fusion protein by cloning into the prokaryotic expression vector pGEX-5X-3. The recombinant Sj-Ts4 protein (rSj-Ts4) was purified and could be recognized by sera of mice infected with S.japonicum. Vaccination of several groups of mice with rSj-Ts4 or rSj-Ts4 incorporated into Freund's complete adjuvant induced high titers of specific IgG antibodies. Two vaccination groups all obtained significant reduction in worm burden (31.36%, 36.80%, P<0.01), compared with the control groups.
为了探究旋毛虫感染动物对血吸虫感染具有高免疫保护作用的分子机制，并为开发抗血吸虫病疫苗提供几种交叉保护性抗原基因，利用感染旋毛虫的小鼠血清对日本血吸虫成虫cDNA文库进行免疫筛选。经过三轮免疫筛选后获得了9个阳性克隆。其中，Sj-Ts4代表日本血吸虫的一个新基因，编码一种由210个氨基酸组成的新蛋白质。该蛋白质的推导分子量为23kD，等电点为7.72。通过克隆到原核表达载体pGEX-5X-3中，将Sj-Ts4表达为谷胱甘肽-S-转移酶（GST）融合蛋白。重组Sj-Ts4蛋白（rSj-Ts4）被纯化，并且能被感染日本血吸虫的小鼠血清识别。用rSj-Ts4或加入弗氏完全佐剂的rSj-Ts4对几组小鼠进行免疫接种，诱导产生了高滴度的特异性IgG抗体。与对照组相比，两个免疫接种组的虫体负荷均显著降低（31.36%，36.80%，P<0.01）。
