Sulima Anna, Savijoki Kirsi, Bień Justyna, Näreaho Anu, Sałamatin Rusłan, Conn David Bruce, Młocicki Daniel
Department of General Biology and Parasitology, Medical University of Warsaw, Warsaw, Poland.
Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.
Front Microbiol. 2018 Jan 15;8:2672. doi: 10.3389/fmicb.2017.02672. eCollection 2017.
Cestodiases are common parasitic diseases of animals and humans. As cestodes have complex lifecycles, hexacanth larvae, metacestodes (including cysticercoids), and adults produce proteins allowing them to establish invasion and to survive in the hostile environment of the host. is the most commonly used model cestode in experimental parasitology. The aims of the present study were to perform a comparative proteomic analysis of two consecutive developmental stages of (cysticercoid and adult) and to distinguish proteins which might be characteristic for each of the stages from those shared by both stages. Somatic proteins of were isolated from 6-week-old cysticercoids and adult tapeworms. Cysticercoids were obtained from experimentally infected beetles, , whereas adult worms were collected from experimentally infected rats. Proteins were separated by GeLC-MS/MS (one dimensional gel electrophoresis coupled with liquid chromatography and tandem mass spectrometry). Additionally protein samples were digested in-liquid and identified by LC-MS/MS. The identified proteins were classified according to molecular function, cellular components and biological processes. Our study showed a number of differences and similarities in the protein profiles of cysticercoids and adults; 233 cysticercoid and 182 adult proteins were identified. From these proteins, 131 were present only in the cysticercoid and 80 only in the adult stage samples. Both developmental stages shared 102 proteins; among which six represented immunomodulators and one is a potential drug target. In-liquid digestion and LC-MS/MS complemented and confirmed some of the GeLC-MS/MS identifications. Possible roles and functions of proteins identified with both proteomic approaches are discussed.
绦虫病是人和动物常见的寄生虫病。由于绦虫具有复杂的生命周期，六钩蚴幼虫、中绦期幼虫（包括似囊尾蚴）和成虫会产生蛋白质，使它们能够在宿主体内的恶劣环境中建立感染并存活。[具体绦虫名称]是实验寄生虫学中最常用的绦虫模型。本研究的目的是对[具体绦虫名称]的两个连续发育阶段（似囊尾蚴和成虫）进行比较蛋白质组学分析，并区分可能是每个阶段特有的蛋白质与两个阶段共有的蛋白质。[具体绦虫名称]的体细胞蛋白从6周龄的似囊尾蚴和成虫绦虫中分离出来。似囊尾蚴从实验感染的甲虫[甲虫名称]中获得，而成虫则从实验感染的大鼠中收集。蛋白质通过GeLC-MS/MS（一维凝胶电泳与液相色谱和串联质谱联用）进行分离。此外，蛋白质样品进行了液相消化，并通过LC-MS/MS进行鉴定。鉴定出的蛋白质根据分子功能、细胞成分和生物学过程进行分类。我们的研究表明，似囊尾蚴和成虫的蛋白质谱存在一些差异和相似之处；共鉴定出233种似囊尾蚴蛋白和182种成虫蛋白。在这些蛋白质中，131种仅存在于似囊尾蚴阶段，80种仅存在于成虫阶段的样品中。两个发育阶段共有102种蛋白质；其中六种代表免疫调节剂，一种是潜在的药物靶点。液相消化和LC-MS/MS补充并证实了一些GeLC-MS/MS的鉴定结果。讨论了两种蛋白质组学方法鉴定出的蛋白质的可能作用和功能。
