Soussi-Yanicostas Nadia, de Castro Fernando, Julliard A Karyn, Perfettini Isabelle, Chédotal Alain, Petit Christine
Unité de Génétique des Déficits Sensoriels, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1968, Institut Pasteur, 25 rue du Dr Roux, F-75724 Paris cedex 15, France.
Cell. 2002 Apr 19;109(2):217-28. doi: 10.1016/s0092-8674(02)00713-4.
The physiological role of anosmin-1, defective in the X chromosome-linked form of Kallmann syndrome, is not yet known. Here, we show that anti-anosmin-1 antibodies block the formation of the collateral branches of rat olfactory bulb output neurons (mitral and tufted cells) in organotypic cultures. Moreover, anosmin-1 greatly enhances axonal branching of these dissociated neurons in culture. In addition, coculture experiments with either piriform cortex or anosmin-1-producing CHO cells demonstrate that anosmin-1 is a chemoattractant for the axons of these neurons, suggesting that this protein, which is expressed in the piriform cortex, attracts their collateral branches in vivo. We conclude that anosmin-1 has a dual branch-promoting and guidance activity, which plays an essential role in the patterning of mitral and tufted cell axon collaterals to the olfactory cortex.
在X染色体连锁型卡尔曼综合征中存在缺陷的anosmin-1的生理作用尚不清楚。在此，我们表明抗anosmin-1抗体可阻断器官型培养物中大鼠嗅球输出神经元（二尖瓣细胞和簇状细胞）侧支的形成。此外，anosmin-1可极大地增强这些解离神经元在培养物中的轴突分支。另外，与梨状皮质或产生anosmin-1的CHO细胞的共培养实验表明，anosmin-1是这些神经元轴突的化学引诱剂，这表明这种在梨状皮质中表达的蛋白质在体内吸引它们的侧支。我们得出结论，anosmin-1具有促进分支和引导的双重活性，这在二尖瓣细胞和簇状细胞轴突侧支向嗅皮质的模式形成中起重要作用。
