Scandalios J G, Sorenson J C, Ott L A
Biochem Genet. 1975 Dec;13(11-12):759-69. doi: 10.1007/BF00484407.
Glutamate oxaloacetic transaminase (L-aspertate: 2-oxoglutarate aminotransferase, E.C. 2.6.1.1; GOT) was found to occur in five distinct electrophoretic forms in different tissue extracts from a number of highly inbred strains of Zea mays L. No major qualitative differences were detected in the various tissues examined, and the isozyme patterns did not undergo changes during temporal development of any given inbred strain. Cell fractionation studies showed one isozyme to be associated with the mitochondria (mGOT), another to be exclusively associated with the soluble fraction (sGOT), and a third to be associated with the glyoxysomes (gGOT). The glyoxysomal form occurs as two electrophoretically distinct variants which exist in different inbred strains of maize. The gGOT variants are under the control of two codiminant alleles (Got1A and Got1B) at the Got1 locus (isozyme5, gGOT). The genetic data and gene dosage effects suggest that GOT in maize is functionally a dimer.
谷氨酸草酰乙酸转氨酶（L-天冬氨酸：2-氧代戊二酸转氨酶，E.C. 2.6.1.1；GOT）在多个高度自交系的玉米（Zea mays L.）不同组织提取物中被发现有五种不同的电泳形式。在所检测的各种组织中未检测到主要的定性差异，并且在任何给定自交系的发育过程中，同工酶模式也没有发生变化。细胞分级分离研究表明，一种同工酶与线粒体相关（mGOT），另一种仅与可溶性部分相关（sGOT），第三种与乙醛酸循环体相关（gGOT）。乙醛酸循环体形式以两种电泳上不同的变体存在，它们存在于玉米的不同自交系中。gGOT变体受Got1位点（同工酶5，gGOT）上的两个共显性等位基因（Got1A和Got1B）控制。遗传数据和基因剂量效应表明，玉米中的GOT在功能上是二聚体。
