Sen S, Payne P I, Osborne D J
Biochem J. 1975 Jun;148(3):381-7. doi: 10.1042/bj1480381.
Incorporation studies with radioactive precursors showed that synthesis of protein and RNA is initiated in germinating embryos of rye within the first hour of imbibition of water. By polyacrylamide-gel fractionations of radioactive nucleic acid components, the appearance of products of transcription of the genome was shown to follow the sequence: heterogeneous (ribonuclease-sensitive) RNA, 4S and 5S RNA by 20min, 31S and 25S rRNA by 40min, and 18S RNA by 60min. "Fingerprint' analysis of T1-ribonuclease digests show that all the large oligonucleotides present in 25S and 18S RNA are present in the 31S species, indicating that 31S RNA is the precursor rRNA molecule to both 25S and 18S RNA. The importance of these early RNA syntheses and in particular the possible template function of the heterogeneous RNA is discussed in relation to the concept of long-lived mRNA and the coding for protein synthesis in the first hours of germination.
利用放射性前体进行的掺入研究表明，在黑麦种子吸水萌发的最初一小时内，蛋白质和RNA的合成即已开始。通过对放射性核酸成分进行聚丙烯酰胺凝胶分级分离，发现基因组转录产物的出现顺序如下：不均一（核糖核酸酶敏感）RNA，20分钟时出现4S和5S RNA，40分钟时出现31S和25S核糖体RNA，60分钟时出现18S RNA。对T1核糖核酸酶消化产物进行的“指纹”分析表明，25S和18S RNA中存在的所有大的寡核苷酸在31S RNA中也都存在，这表明31S RNA是25S和18S RNA的前体核糖体RNA分子。结合长寿命信使RNA的概念以及萌发最初几小时内蛋白质合成的编码问题，讨论了这些早期RNA合成的重要性，特别是不均一RNA可能的模板功能。
