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种子吸胀后的蛋白质合成 III. 氨基酸掺入的动力学 核糖体激活和多核糖体形成。

Protein Synthesis in Imbibed Seeds III. Kinetics of Amino Acid Incorporation Ribosome Activation, and Polysome Formation.

机构信息

Market Quality Research Division, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland.

出版信息

Plant Physiol. 1966 Sep;41(7):1167-72. doi: 10.1104/pp.41.7.1167.

Abstract

The kinetics of development of protein-synthesizing capacity in the imbibing wheat embryo, were studied both in vivo and in vitro. During the first 30 minutes of imbibition protein-synthesizing capacity rises rapidly, lagging about 10 minutes behind water uptake. This rise in synthesizing capacity is accompanied by an increase in polysome content. As imbibition continues, both protein-synthesizing capacity and polysome content increase. With embryos from aged seed, the rate of protein synthesis is initially limited by another, presumably nonribosomal, reaction.

摘要

本文研究了吸胀小麦胚中蛋白质合成能力的发展动力学,分别进行了体内和体外研究。在吸胀的最初 30 分钟内,蛋白质合成能力迅速上升,比吸水滞后约 10 分钟。这种合成能力的上升伴随着多核糖体含量的增加。随着吸胀的继续,蛋白质合成能力和多核糖体含量都增加。对于来自老化种子的胚,蛋白质合成的速率最初受到另一种推测是非核糖体反应的限制。

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