Wan Yongqin, Angleson Joseph K, Kutateladze Andrei G
Contribution from the Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 80208, USA.
J Am Chem Soc. 2002 May 22;124(20):5610-1. doi: 10.1021/ja016874i.
A molecular dithiane-based approach to synthesis of novel photolabile phospholipids is developed. These lipids are used in formulations with egg-POPC and cholesterol to prepare light-sensitive liposomes. Irradiation of such liposomes in PBS buffer (medium pressure mercury lamp, Pyrex filter, lambda > 300 nm) significantly increases the bilayer permeability and accelerates the release of entrapped small organic molecules by an order of magnitude. A simple assay, based on (1)H or (19)F PFG NMR measurements of diffusion coefficients, is developed to monitor light-induced unloading of the probe molecules.
开发了一种基于分子二硫烷的新型光不稳定磷脂合成方法。这些脂质用于与鸡蛋-POPC和胆固醇的配方中，以制备光敏脂质体。在PBS缓冲液中照射此类脂质体（中压汞灯，派热克斯滤光片，λ> 300 nm）可显著提高双层膜通透性，并使包裹的小有机分子的释放速度加快一个数量级。开发了一种基于扩散系数的（1）H或（19）F PFG NMR测量的简单测定法，以监测光诱导的探针分子卸载。
