Blackwell Nathan M, Else Kathryn J
School of Biological Sciences, University of Manchester, Manchester, UK.
Parasite Immunol. 2002 Apr;24(4):203-11. doi: 10.1046/j.1365-3024.2002.00452.x.
The serum parasite-specific antibody responses of different mouse strains infected with Trichuris muris reflect the nature of the T-helper response mounted by the host, in that resistant Th2-responding strains, such as BALB/K, produce immunoglobulin (Ig)G1 and susceptible predominantly Th1-responding strains, such as AKR, produce IgG2a and IgG1. However, the kinetics of antibody production in the sera, as determined by enzyme-linked immunosorbent assay, do not reflect infection status in that resistant strains can expel their worm burdens before antibodies are detectable in the sera. Here, we show that parasite-specific antibody production by in vitro lipopolysaccharide-stimulated mesenteric lymph node cells (MLN) not only correlate with serum antibody isotypes, but also follow expulsion kinetics. Additionally, the antibody levels seen locally match changes in absolute B220+ cell numbers in the MLN (determined by flow cytometry) and changes in MLN parasite-specific plasma cells in the MLN (determined by ELISPOT). These results show that B cell responses are tightly regulated locally in both resistant and susceptible strains of mice infected with T. muris.
感染毛首鞭形线虫的不同小鼠品系的血清寄生虫特异性抗体反应反映了宿主产生的辅助性T细胞反应的性质，即抗性的Th2反应性品系（如BALB/K）产生免疫球蛋白（Ig）G1，而易感的主要为Th1反应性品系（如AKR）产生IgG2a和IgG1。然而，通过酶联免疫吸附测定法测定的血清中抗体产生的动力学并不反映感染状态，因为抗性品系在血清中可检测到抗体之前就能排出其体内的虫负荷。在此，我们表明，体外脂多糖刺激的肠系膜淋巴结细胞（MLN）产生的寄生虫特异性抗体不仅与血清抗体亚型相关，而且遵循排出动力学。此外，局部观察到的抗体水平与MLN中绝对B220+细胞数量的变化（通过流式细胞术测定）以及MLN中MLN寄生虫特异性浆细胞的变化（通过酶联免疫斑点测定法测定）相匹配。这些结果表明，在感染毛首鞭形线虫的抗性和易感小鼠品系中，B细胞反应在局部都受到严格调控。
