Koyama K, Ito Y
Department of Parasitology, Kitasato University School of Medicine, Kanagawa, Japan.
Parasite Immunol. 1996 May;18(5):257-63. doi: 10.1046/j.1365-3024.1996.d01-92.x.
A comparison of immune responses to infection between groups of B10.BR mice infected with different strains of T. muris, S strain (isolated in Sobreda, Portugal), E strain (isolated in Edinburgh), and E-J strain (originally E strain, which has been maintained in our laboratory, Japan), was performed. In mice infected with E and E-J strains, most of the worms were expelled by day 32 after infection, though the expulsion was faster in E-J strain-infected mice. In contrast, no expulsion was observed in S strain-infected mice by day 32 and egg production occurred on day 32. IL-4 production occurred in concanavalin A (Con-A)-stimulated mesenteric lymph node cells (MLNC) from B10.BR mice infected with E and E-J strains, whereas no IL-4 production was observed in S strain-infected mice. IL-4 production did not occur in normal mice. In comparison with normal mice, high levels of IFN-gamma production by Con A-stimulated MLNC were detected in mice infected with every strain of T. muris. IFN-gamma production in S strain-infected mice was greater, occurred earlier and was more persistent than in mice infected with E and E-J strains. IgG1 and IgG2a antibodies to T. muris excretory/ secretory antigens were observed in B10.BR mice infected with every strain of T. muris. Antibody production showed similar kinetics. These differences in the expulsion kinetics and IL-4 production in B10.BR mice infected with S, E, and E-J strains suggest the involvement of IL-4 in protection against T. muris infection, and confirm the previous conclusion by Else et al.
对感染不同菌株的B10.BR小鼠组之间针对感染的免疫反应进行了比较，这些菌株包括鼠鞭虫（T. muris）的S株（在葡萄牙索布雷达分离）、E株（在爱丁堡分离）和E-J株（原E株，在日本我们实验室保存）。在感染E株和E-J株的小鼠中，大多数蠕虫在感染后第32天被排出，不过在感染E-J株的小鼠中排出速度更快。相比之下，在感染S株的小鼠中，到第32天未观察到蠕虫排出，且在第32天出现了产卵。感染E株和E-J株的B10.BR小鼠经伴刀豆球蛋白A（Con-A）刺激的肠系膜淋巴结细胞（MLNC）中产生白细胞介素-4（IL-4），而在感染S株的小鼠中未观察到IL-4产生。正常小鼠中不产生IL-4。与正常小鼠相比，在感染每种鼠鞭虫菌株的小鼠中均检测到Con A刺激的MLNC产生高水平的γ干扰素（IFN-γ）。感染S株的小鼠中IFN-γ产生量更大、出现更早且比感染E株和E-J株的小鼠更持久。在感染每种鼠鞭虫菌株的B10.BR小鼠中均观察到针对鼠鞭虫排泄/分泌抗原的IgG1和IgG2a抗体。抗体产生表现出相似的动力学。感染S、E和E-J株的B10.BR小鼠在排出动力学和IL-4产生方面的这些差异表明IL-4参与了抵抗鼠鞭虫感染的保护作用，并证实了Else等人先前的结论。
