Travers Susan P
Section of Oral Biology, College of Dentistry, The Ohio State University, Columbus, Ohio 43218-2357, USA.
Am J Physiol Regul Integr Comp Physiol. 2002 Jun;282(6):R1798-810. doi: 10.1152/ajpregu.00590.2001.
The present experiment investigated Fos-like immunoreactivity (FLI) in the nucleus of the solitary tract (NST) after intraoral infusions of 0.1 M citric acid, 0.3 M NaCl, and 0.3-30 mM quinine monohydrochloride (QHCl) in awake, behaving rats. Increases in QHCl concentration produced increases in the numbers of FLI-labeled neurons in the rostral part of the intermediate (i(r)) and rostral (r) NST, but the topographic distribution of FLI was consistent across QHCl concentrations and distinctive compared with effects of citric acid. Quinine elicited FLI concentrated in the medial third of the nucleus; acid elicited more broadly distributed FLI concentrated farther laterally. Surprisingly, in contrast to QHCl and citric acid, NaCl produced FLI that was indistinguishable from that produced by water. Although the functional significance of these patterns is unknown, citric acid and QHCl are nonpreferred stimuli but produced different oromotor behaviors. QHCl (30 mM) elicited approximately 3.2 times as many gapes as citric acid (0.1 M), and acid elicited more ingestive responses. Parallel differences in FLI expression suggest that different NST regions may have distinctive roles in triggering oromotor behaviors.
本实验研究了在清醒、活动的大鼠口腔内输注0.1M柠檬酸、0.3M氯化钠和0.3 - 30mM盐酸奎宁（QHCl）后，孤束核（NST）中Fos样免疫反应性（FLI）的情况。QHCl浓度的增加导致中间部（i(r)）和吻侧（r）NST吻侧部分FLI标记神经元数量增加，但FLI的拓扑分布在不同QHCl浓度下是一致的，且与柠檬酸的作用相比具有明显差异。奎宁引起的FLI集中在核的内侧三分之一；酸引起的FLI分布更广泛，集中在更外侧。令人惊讶的是，与QHCl和柠檬酸不同，氯化钠产生的FLI与水产生的FLI无法区分。尽管这些模式的功能意义尚不清楚，但柠檬酸和QHCl是非优选刺激物，但产生了不同的口面部运动行为。QHCl（30mM）引起的张口次数约为柠檬酸（0.1M）的3.2倍，且酸引起更多的摄食反应。FLI表达的平行差异表明，不同的NST区域可能在触发口面部运动行为中具有独特作用。
