Travers J B, Urbanek K, Grill H J
Oral Biology, Ohio State University, Columbus, Ohio 43210, USA.
Am J Physiol. 1999 Aug;277(2):R384-94. doi: 10.1152/ajpregu.1999.277.2.R384.
The present study compared the distribution of Fos-like immunoreactivity (FLI) following intraoral stimulation with quinine monohydrochloride (QHCl) in awake intact rats to the pattern obtained in chronic supracollicular decerebrate (CD) rats. Because the behavioral rejection response to QHCl is evident in the CD rat, it was hypothesized that the pattern of FLI in the lower brain stem should be similar in both groups. Overall, the distribution of FLI in the brain stem was quite similar in both intact and CD groups, and QHCl stimulation increased FLI in the rostral (gustatory) nucleus of the solitary tract, the parabrachial nucleus (PBN), and the lateral reticular formation (RF) compared with an unstimulated control group. The CD group differed from the intact group, however, with a trend toward less FLI in the RF and a shift in the pattern of label away from the external subdivision of the PBN. CD rats also had increased FLI in the caudal nucleus of the solitary tract, with or without intraoral infusions. The distribution of QHCl-induced FLI in the brain stem of intact rats thus indicates both local sensorimotor processing as well as the influence of forebrain structures.
本研究比较了清醒完整大鼠经口给予盐酸奎宁（QHCl）刺激后Fos样免疫反应（FLI）的分布与慢性上丘去大脑（CD）大鼠的模式。由于CD大鼠对QHCl的行为排斥反应明显，因此推测两组大鼠脑干下部的FLI模式应相似。总体而言，完整组和CD组脑干中FLI的分布相当相似，与未刺激的对照组相比，QHCl刺激增加了孤束核吻侧（味觉）核、臂旁核（PBN）和外侧网状结构（RF）中的FLI。然而，CD组与完整组不同，RF中的FLI有减少的趋势，且标记模式从PBN的外侧亚区转移。无论是否经口注入，CD大鼠孤束核尾侧核中的FLI也增加。因此，完整大鼠脑干中QHCl诱导的FLI分布既表明了局部感觉运动处理，也表明了前脑结构的影响。
