伯氏疏螺旋体假定脂蛋白基因的DNA微阵列评估
DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes.
作者信息
Liang Fang Ting, Nelson F Kenneth, Fikrig Erol
机构信息
Section of Rheumatology, Department of Internal Medicine, School of Medicine, Yale University, New Haven, Connecticut 06520-8031, USA.
出版信息
Infect Immun. 2002 Jun;70(6):3300-3. doi: 10.1128/IAI.70.6.3300-3303.2002.
Abstract
A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.
摘要
一个包含137个伯氏疏螺旋体B31假定脂蛋白基因片段的DNA微阵列被用于检测莱姆病螺旋体。来自严格意义上的伯氏疏螺旋体B31、297和N40；伽氏疏螺旋体IP90；以及阿氏疏螺旋体P/Gau的DNA被荧光标记并与该微阵列杂交，显示了各个假定脂蛋白基因在不同基因种间的保守程度。这些数据表明，DNA微阵列可以全面检测编码伯氏疏螺旋体脂蛋白的基因。
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