Banga I
Atherosclerosis. 1975 Nov-Dec;22(3):533-41. doi: 10.1016/0021-9150(75)90031-3.
(1) Annulus fibrosus and nucleus pulposus of human intervertebral discs at different degrees of atherosclerosis were disintegrated by elastase. (2) The material disintegrated by elastase -- called elastolysate -- could be separated into hydrophobic (apolar) and hydrophilic (polar) peptides. Parallel with the degree of atherosclerosis the amount of hydrophobic peptides increased, whereas that of the hydrophilic peptides decreased. (3) In annulus fibrosus and nucleus pulposus two kinds of fluorescence maxima were measured. The one, A:F 350:405, is known as fluorescence maxima of elastin- and collagen-peptides. The other, A:F 410:470, is related to a similar substance called atherofluorescent component (AFC), which has been isolated before from the plaques of atherosclerotic aorta. This substance accumulates mainly in nucleus pulposus and resembles lipofuscin-like bodies. (4) These bodies show a positive reaction with thiobarbituric acid, giving a red coloration characteristic for malondialdehyde. In nucleus pulposus the amount of lipofuscin-like substances is much greater than in annulus fibrosus. (5) The hydrophilic peptides, although they show the same fluorescence maxima as the hydrophobic peptides, do not give any reaction with thiobarbituric acid. It is supposed that in these cases the cross-linked protein contains instead of malondialdehyde other reactive aldehydes.
(1) 用弹性蛋白酶分解不同程度动脉粥样硬化的人椎间盘纤维环和髓核。(2) 经弹性蛋白酶分解的物质——称为弹性溶解产物——可分离成疏水（非极性）和亲水（极性）肽段。随着动脉粥样硬化程度的增加，疏水肽段的量增加，而亲水肽段的量减少。(3) 在纤维环和髓核中检测到两种荧光最大值。一种是A:F 350:405，已知是弹性蛋白和胶原蛋白肽段的荧光最大值。另一种是A:F 410:470，与一种类似的物质有关，称为动脉粥样荧光成分（AFC），此前已从动脉粥样硬化主动脉斑块中分离出来。这种物质主要积聚在髓核中，类似于脂褐素样小体。(4) 这些小体与硫代巴比妥酸呈阳性反应，产生丙二醛特有的红色。在髓核中，脂褐素样物质的量比纤维环中多得多。(5) 亲水肽段虽然与疏水肽段有相同的荧光最大值，但与硫代巴比妥酸不发生任何反应。据推测，在这些情况下，交联蛋白中含有的不是丙二醛，而是其他反应性醛类。
