Riordan J R, Gower I
Biochim Biophys Acta. 1975 Dec 5;411(2):393-8. doi: 10.1016/0304-4165(75)90322-0.
The same three low molecular weight copper-binding proteins which have been purified from the soluble fractions of Cu2+-loaded livers (200 mug Cu2+ per g wet weight) were also found to be present in livers of normal rats (4 mug Cu2+ per g wet weight) but in much smaller quantities. Therefore, Cu2+ loading enhances the amount of preexisting proteins rather than inducing the synthesis of new proteins. Amino acid analyses of each of the three showed them to be different from the metallothioneins which are present on loading with other trace metals including Cd2+ and Zn2+.
从铜离子负载肝脏（每克湿重含200微克铜离子）的可溶性部分中纯化出的三种低分子量铜结合蛋白，也存在于正常大鼠肝脏（每克湿重含4微克铜离子）中，但含量要少得多。因此，铜离子负载增加了现有蛋白质的量，而不是诱导新蛋白质的合成。对这三种蛋白进行氨基酸分析，结果表明它们与金属硫蛋白不同，金属硫蛋白是在负载包括镉离子和锌离子在内的其他痕量金属时出现的。
