大鼠锌状态对铜诱导的金属硫蛋白合成与降解的影响。
Effect of zinc status of rats on the synthesis and degradation of copper-induced metallothioneins.
作者信息
Bremner I, Hoekstra G, Davies N T, Young B W
出版信息
Biochem J. 1978 Sep 15;174(3):883-92. doi: 10.1042/bj1740883.
Abstract
Injection of Zn2+-adequate and Zn2+-deficient rats with Cu2+ stimulated the incorporation of l-[35S]cysteine into a low-molecular-weight Cu2+-binding protein in both liver and kidney. No significant incorporation of l-[4,5-3H]leucine into this protein occurred, confirming the previous claim that it was metallothionein and not some other leucine-rich protein. The half-life of the protein was found to be 16.9 +/- 1.0 (S.E.)h in the liver of Zn2+-adequate rats but only 12.3 +/- 0.5h in Zn2+-deficient animals. The degradation rate of the metallothionein was similar to the rate of disappearance of Cu2+ and Zn2+ from the protein, indicating that the release of mental from the protein and its catabolism occurred simultaneously. There was no significant difference in the half-lives of the hepatic or renal copper-thioneins in Zn2+-adequate rats.
摘要
给锌充足和锌缺乏的大鼠注射铜离子，会刺激肝脏和肾脏中l-[35S]半胱氨酸掺入一种低分子量的铜离子结合蛋白。没有观察到l-[4,5-3H]亮氨酸显著掺入该蛋白，这证实了之前的说法，即它是金属硫蛋白而不是其他富含亮氨酸的蛋白。在锌充足的大鼠肝脏中，该蛋白的半衰期为16.9±1.0（标准误）小时，而在锌缺乏的动物中仅为12.3±0.5小时。金属硫蛋白的降解速率与铜离子和锌离子从该蛋白中消失的速率相似，表明金属从蛋白中的释放及其分解代谢同时发生。锌充足的大鼠肝脏或肾脏中铜硫蛋白的半衰期没有显著差异。
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