五味子超临界二氧化碳提取物的液相色谱分析
Liquid chromatographic analysis of supercritical carbon dioxide extracts of Schizandra chinensis.
作者信息
Bártlová Milena, Opletal Lubomír, Chobot Vladimír, Sovová Helena
机构信息
Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, Prague.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Apr 25;770(1-2):283-9. doi: 10.1016/s1570-0232(01)00634-1.
Six major lignans (schizandrin, gomisin A, deoxyschizandrin, y-schizandrin, gomisin N, wuweizisu C) in the caulomas and leaves of Schizandra chinensis (Turcz.) Baill., and cinnamic acid in the leaves of the plant, were quantitatively analysed by high-performance liquid chromatography in reversed-phase mode with UV detection. Resolution of the determined lignans was evaluated for two multistep gradients applied. Samples for HPLC analysis were prepared by extraction with supercritical carbon dioxide at pressures of 20-27 MPa and temperatures of 40-60 degrees C. Kinetics of the extraction of individual components was measured and simulated with a model.
采用反相高效液相色谱法结合紫外检测，对五味子（Schizandra chinensis (Turcz.) Baill.）茎皮和叶片中的六种主要木脂素（五味子素、戈米辛A、脱氧五味子素、γ-五味子素、戈米辛N、五味子酯甲）以及该植物叶片中的肉桂酸进行了定量分析。针对两种应用的多步梯度，评估了所测定木脂素的分离度。通过在20 - 27 MPa压力和40 - 60℃温度下用超临界二氧化碳萃取来制备用于高效液相色谱分析的样品。测量了各组分的萃取动力学并用模型进行了模拟。
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