Murakami Takahiro
Division of Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 2002 Apr;64(4):321-3. doi: 10.1292/jvms.64.321.
Nucleotide sequences of cytochrome b/tRNA/D-loop region on mitochondrial DNA of mustelids feces were compared to identify species. PCR amplification of target sequence for 47 (24.9%) feces and species identification of five feces (2.6%) out of 189 feces, collected at several study sites in Hokkaido, were successful. Species of three feces were Martes zibellina and those of other two feces were Martes melampus and Mustela itatsi. The low success rate of identification appeared to be due to failure of PCR amplification by inhibitors in feces. It was suggested that the method used in this study was useful for not only identify mustelids species, but also analyzing their genetic relationships.
为了鉴定物种，对鼬科动物粪便线粒体DNA上的细胞色素b/tRNA/D环区域的核苷酸序列进行了比较。在北海道的几个研究地点收集的189份粪便中，47份（24.9%）粪便的目标序列PCR扩增成功，5份（2.6%）粪便的物种鉴定成功。三份粪便的物种是紫貂，另外两份粪便的物种是黄喉貂和黄鼬。鉴定成功率较低似乎是由于粪便中的抑制剂导致PCR扩增失败。结果表明，本研究中使用的方法不仅有助于鉴定鼬科动物物种，还能分析它们的遗传关系。
