Davidson Bruce C, Giangregorio Alfredo
University of the Witwatersrand Medical School, Parktown, South Africa.
Anticancer Res. 2002 Mar-Apr;22(2A):893-8.
Cell culture has been used for many years, mainly using transformed cells, but in some instances normal cells. Generally, the more unsaturated fatty acids were most efficient at limiting cell growth. These fatty acids were usually more efficient at cell growth limitation and cell killing when used with transformed cells, compared to normal controls. Different species of mammals exhibit different capability to metabolise fatty acids, especially polyunsaturated fatty acids. The enzymes for such metabolism are largely cytosolic and mitochondrial, and are not found in erythrocytes. We have maintained erythrocytes from three species (rat, human and cat) in culture and exposed them to mixtures of fatty acids mimicking the composition of seven dietary oils. The mixtures induced varying degrees of erythrocyte death, generally depending on the type and amount of polyunsaturated fatty acids present in, and the concentration of the mixtures. Comparing the three species, the rat cells were generally the most susceptible to the effects of the fatty acids, with either human or cat least susceptible depending on the specific fatty acid mixture. Erythrocytes cannot metabolise fatty acids and hence are not able to remove the mixture dosed, and any effects are most likely to relate to changes in the erythrocyte membrane phosphoglyceride bilayer structure.
细胞培养已经使用多年，主要使用转化细胞，但在某些情况下也使用正常细胞。一般来说，不饱和程度越高的脂肪酸在限制细胞生长方面效率最高。与正常对照相比，这些脂肪酸与转化细胞一起使用时，在限制细胞生长和杀死细胞方面通常更有效。不同种类的哺乳动物对脂肪酸，尤其是多不饱和脂肪酸的代谢能力不同。这种代谢的酶主要存在于胞质溶胶和线粒体中，在红细胞中不存在。我们在培养中维持了来自三个物种（大鼠、人类和猫）的红细胞，并将它们暴露于模拟七种食用油成分的脂肪酸混合物中。这些混合物引起了不同程度的红细胞死亡，一般取决于混合物中多不饱和脂肪酸的类型和含量以及混合物的浓度。比较这三个物种，大鼠细胞通常对脂肪酸的影响最敏感，人类或猫细胞最不敏感，具体取决于特定的脂肪酸混合物。红细胞不能代谢脂肪酸，因此无法清除所给予的混合物，任何影响很可能与红细胞膜磷酸甘油酯双层结构的变化有关。
