Davidson B C, Girao L A, Giangregorio A, Murphy J
Department of Medical Biochemistry, University of the Witwatersrand Medical School, Johannesburg, South Africa.
Anticancer Res. 1991 Jan-Feb;11(1):267-72.
Normal human skin fibroblasts and cells from 2 benign (3T3 and 3T6B) and 1 malignant (3T6D) fibroblast lines were grown in the presence of C20 fatty acids in the concentration range 0-100 mg/l culture medium. The culture medium consisted of 10% FCS and 90% DMEM, the cells were dosed with various concentrations of fatty acid in ethanol as carrier 24 hours post-trypsinisation, and the viability determined using Trypan Blue exclusion after a further 48 hours. The threshold between cytostatic and cytotoxic effects was considered to be that concentration of fatty acid which limited cell number to that of the controls at the end of the post-trypsinisation recovery period. All the fatty acids exhibited growth limitation potential, to a greater or lesser extent, which varied dependent on the degree of unsaturation of the molecule and transformation of the fibroblasts. In general, the effects appeared more likely to be related to the role of fatty acids in phosphoglycerides, rather related to the role of fatty acids in phosphoglycerides, rather than via eicosanoid metabolism.
将正常人皮肤成纤维细胞以及来自2种良性（3T3和3T6B）和1种恶性（3T6D）成纤维细胞系的细胞，在含有浓度范围为0 - 100mg/L培养基的C20脂肪酸存在的情况下进行培养。培养基由10%胎牛血清（FCS）和90% Dulbecco改良 Eagle培养基（DMEM）组成，在胰蛋白酶消化后24小时，将细胞用不同浓度的以乙醇为载体的脂肪酸给药，并在另外48小时后使用台盼蓝排斥法测定细胞活力。细胞生长抑制和细胞毒性作用之间的阈值被认为是在胰蛋白酶消化后恢复期结束时，使细胞数量限制在与对照相同水平的脂肪酸浓度。所有脂肪酸均表现出不同程度的生长限制潜力，其程度取决于分子的不饱和度以及成纤维细胞的转化情况。一般来说，这些作用似乎更可能与脂肪酸在磷酸甘油酯中的作用有关，而不是通过类花生酸代谢。
