Svoboda Beatrice, Getoff Nikola
Ludwig Boltzmann Institute for Radiation Chemistry and Radiation Biology, University of Vienna, Wien, Austria.
Anticancer Res. 2002 Mar-Apr;22(2A):949-52.
The radiation-induced effect of Vitamin B6 (Vit. B6) on mitomycin C (MMC) was investigated by using Escherichia coli bacteria AB 1157 as a model in air-free media as well as in media saturated with nitrous oxide (N20) or air, respectively. In all three types of media Vit.B6 showed cytostatic abilities. The highest synergistic effect of Vit.B6 on MMC was observed in an air-free environment. It decreased 2.8-fold in aerated solution and showed the opposite effect in solutions saturated with N20. The addition of Vitamin C (Vit.C) to the Vit.B6/MMC-mixture in air-free media reduced the MMC-efficiency by a factor of 3.6, whereas the presence of air led to a MMC enhancement of 1.6-fold. Considerations based on the involvement of the primary transients of water radiolysis were made in order to explain the observed effects.
以大肠杆菌AB 1157为模型，分别在无氧培养基以及充满一氧化二氮（N20）或空气的培养基中，研究了维生素B6（Vit. B6）对丝裂霉素C（MMC）的辐射诱导效应。在所有三种类型的培养基中，Vit.B6均表现出细胞生长抑制能力。在无氧环境中观察到Vit.B6对MMC的协同效应最高。在通气溶液中其协同效应降低了2.8倍，而在充满N20的溶液中则表现出相反的效应。在无氧培养基中向Vit.B6/MMC混合物中添加维生素C（Vit.C），会使MMC的效率降低3.6倍，而在有空气的情况下，MMC的效率会提高1.6倍。基于水辐射分解的初级瞬态参与情况进行了相关考量，以解释所观察到的效应。
