Baines A D, Timanoff M, Wong J, Paul W
Can J Physiol Pharmacol. 1975 Oct;53(5):704-8. doi: 10.1139/y75-098.
The construction of a simple device for continuous monitoring and recording of plasma indocyanine green concentration in small animals is described. The apparatus is designed to hold a vascular bed such as the ear or the omentum and to transilluminate it with white light from a fiber optic bundle. The transmitted light is divided by a dichroic mirror. Appropriate filters select a narrow band of frequencies from each segment. There are two photovoltaic cells, one sensitive to the dye and the other insensitive to it. Both are approximately equally reactive to changes in transilluminence caused by changes in blood content or hematocrit and are relatively insensitive to changes in oxygen saturation of the transilluminated blood. Reproducible estimates of cardiac output have been obtained with the injection of 300 mug indocyanine green in a volume of 20 mul with the densitometer applied to the small intestine or to the ear of the animal. The device responds linearly to increasing plasma indocyanine green concentrations and can be calibrated with less than 0.1 ml of blood.
本文描述了一种用于连续监测和记录小动物血浆吲哚菁绿浓度的简易装置的构建。该装置旨在容纳诸如耳朵或网膜之类的血管床，并用来自光纤束的白光对其进行透照。透射光由二向色镜分光。适当的滤光片从每个波段中选择一个窄频带。有两个光伏电池，一个对染料敏感，另一个对染料不敏感。两者对因血液含量或血细胞比容变化引起的透照变化反应大致相同，并且对透照血液的氧饱和度变化相对不敏感。通过向动物的小肠或耳朵应用密度计，以20微升的体积注射300微克吲哚菁绿，已获得可重复的心输出量估计值。该装置对血浆吲哚菁绿浓度的增加呈线性响应，并且可以用少于0.1毫升的血液进行校准。
