Lim B H, Rahmah N, Afifi S A, Ramli A, Mehdi R
Department of Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan.
Med J Malaysia. 2001 Dec;56(4):491-6.
A total of 1,134 finger-pricked blood samples were collected from residents of Setiu, Terengganu. A drop of blood was used to make thick blood smear and about four drops were used for obtaining serum. The smears were stained and examined by the State Vector Control Unit in Kuala Terengganu, while the serum samples were tested for specific IgG4 antibodies to a novel recombinant antigen using Brugia-Elisa. Prevalence of filariasis in these areas were found to be 0.26% (3/1,134) using thick blood smear examination and 2.47% (28/1,134) using Brugia-Elisa, thus demonstrating the greater sensitivity of the latter test. In addtion, Brugia-Elisa showed a high level of specificity (97.8%, 1,106/1,131) when compared to thick blood smear examination.
共从登嘉楼州色蒂乌的居民中采集了1134份手指刺破的血样。一滴血用于制作厚血涂片，约四滴血用于获取血清。涂片由瓜拉丁加奴州的病媒控制单位进行染色和检查，而血清样本则使用布鲁吉亚酶联免疫吸附测定法检测针对一种新型重组抗原的特异性IgG4抗体。通过厚血涂片检查发现这些地区的丝虫病患病率为0.26%（3/1134），使用布鲁吉亚酶联免疫吸附测定法的患病率为2.47%（28/1134），从而证明了后一种检测方法具有更高的敏感性。此外，与厚血涂片检查相比，布鲁吉亚酶联免疫吸附测定法显示出较高的特异性（97.8%，1106/1131）。
