Li Xiao, Pu Quan, Liu Yizhi, Shi Jun, Tao Ying, Chang Chunkang, Jiang Qinyan, Huang Wei
Department of Hematology of the Sixth Hospital, Shanghai 200233, China.
Zhonghua Xue Ye Xue Za Zhi. 2002 Jan;23(1):27-9.
To investigate the total in situ apoptotic cell number and the apoptotic situation in erythroid cell and megakaryocytes in patients with myelodysplastic syndromes (MDS).
Apoptosis cell number and the apoptotic situation of erythroid cell and megakaryocytes were analysed on cold embedded bone marrow sections from 25 MDS patients by DNA in situ end labelling (ISEL)/alkaline phosphatase anti-alkaline phosphatase (APAAP) double stained techniques. Fourteen cases of iron deficiency anemia (IDA) were taken as control.
Mean apoptotic cell numbers in MDS and control group were (39.44 +/- 29.34)/mm(2) and (13.43 +/- 8.39)/mm(2) respectively (P < 0.01). RA/RAS subtypes had a higher apoptosis ratio (47.56 +/- 32.86/mm(2)) than that in RAEB/RAEB-t subtypes (21.87 +/- 13.65/mm(2)) (P < 0.05). Double staining showed similar apoptosis percentage in erythroid cell and megakaryocytes in MDS patients comparing with that of controls (P > 0.05). Some apoptotic cells showing erythroid or megakaryocytic morphologic characteristics expressed no cluster differentiation antigen.
Overapoptosis existed in MDS, RA/RAS group had a higher apoptosis ratio than RAEB/RAEB-t group. No obvious increased apoptosis in erythroid cell and megakaryocytes was observed in MDS perhaps due to the loss of surface antigens in later stages of apoptotic cells.
探讨骨髓增生异常综合征（MDS）患者原位凋亡细胞总数以及红系细胞和巨核细胞的凋亡情况。
采用DNA原位末端标记（ISEL）/碱性磷酸酶抗碱性磷酸酶（APAAP）双重染色技术，分析25例MDS患者经冷包埋的骨髓切片中凋亡细胞数量以及红系细胞和巨核细胞的凋亡情况。选取14例缺铁性贫血（IDA）患者作为对照。
MDS组和对照组的平均凋亡细胞数分别为（39.44±29.34）/mm²和（13.43±8.39）/mm²（P<0.01）。RA/RAS亚型的凋亡率（47.56±32.86/mm²）高于RAEB/RAEB - t亚型（21.87±13.65/mm²）（P<0.05）。双重染色显示，MDS患者红系细胞和巨核细胞的凋亡百分比与对照组相似（P>0.05）。一些呈现红系或巨核细胞形态特征的凋亡细胞不表达簇分化抗原。
MDS存在过度凋亡，RA/RAS组的凋亡率高于RAEB/RAEB - t组。MDS患者红系细胞和巨核细胞未观察到明显的凋亡增加，可能是由于凋亡细胞后期表面抗原丢失所致。
