[汉坦病毒与汉城病毒株之间的初步基因重配]

[A preliminary genetic reassortment between Hantaan virus and Seoul virus strains].

作者信息

Kang Wenzhen, Huang Changxing, Bai Xuefan, Yang Weisong, Li Guangyu

机构信息

Department of Infectious Diseases, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2002 Feb;23(1):46-9.

PMID:12015110

Abstract

OBJECTIVE

To determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS).

METHODS

Mixed infections were initiated in tissue culture, using Hantaan virus strain 76 - 118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments (L, M, S) of each strain.

RESULTS

Most of the progeny virus plaques (68.19% of 44) had parental genotype of 76 - 118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid.

CONCLUSION

Genetic reassortment can occur between Hantaan virus and Seoul virus strains.

摘要

目的

确定导致肾综合征出血热（HFRS）的汉坦病毒和汉城病毒之间基因重配的频率及特征。

方法

在组织培养中引发混合感染，使用汉坦病毒76 - 118株和汉城病毒SR - 11株。通过多重逆转录聚合酶链反应（multiplex RT-PCR），使用针对每个毒株单个基因组片段（L、M、S）的特异性引物，挑选出潜在的重配病毒蚀斑。

结果

大多数子代病毒蚀斑（44个中的68.19%）具有76 - １18株或SR - 11株的亲代基因型，而44个蚀斑中有2个具有混合基因型，产生了两个亲代毒株同一段基因组的逆转录聚合酶链反应条带。在44个测试的子代蚀斑中，68.19%检测到重配病毒，涉及M和S片段。此外，约4.55%的子代病毒蚀斑似乎含有源自两个亲代病毒株的S或M片段，表明它们是二倍体。