Avsic-Zupanc T, Poljak M, Furlan P, Kaps R, Xiao S Y, Leduc J W
Institute of Microbiology, Medical Faculty of Ljubljana, Slovenia.
Am J Trop Med Hyg. 1994 Oct;51(4):393-400.
Hemorrhagic fever with renal syndrome (HFRS) has been serologically confirmed in Slovenia during the last seven years. There is evidence that three hantaviruses (Hantaan, Puumala, and a newly described form termed Dobrava) circulate simultaneously in this area. Recently, a hantavirus was isolated from the urine and brain tissue of a fatal case of HFRS. Positive immunofluorescent reactions with reference human sera and monoclonal antibodies were first recognized after the second cell culture passage. Extensive cross-reactivity between our isolate and prototype Hantaan virus, strain 76-118, and Hantaan-like isolates from the former Yugoslavia, Fojnica and Plitvice, was revealed by enzyme-linked immunosorbent assay with specific rat antisera. The reaction pattern of the isolate was similar to the prototype Hantaan virus by indirect immunofluorescent assay with a panel of monoclonal antibodies. Furthermore, the specificity of the isolates was confirmed by analysis of polymerase chain reaction products of this virus with five restriction endonucleases. This appears to be the first isolation of a strain of prototype Hantaan virus from a fatal case of HFRS in Europe.
在过去七年中，斯洛文尼亚经血清学确诊存在肾综合征出血热（HFRS）。有证据表明，三种汉坦病毒（汉滩病毒、普马拉病毒以及一种新发现的名为多布拉瓦病毒的类型）在该地区同时传播。最近，从一例HFRS死亡病例的尿液和脑组织中分离出一种汉坦病毒。在第二次细胞培养传代后，首次通过参考人血清和单克隆抗体识别出阳性免疫荧光反应。通过使用特异性大鼠抗血清的酶联免疫吸附测定法，揭示了我们分离出的病毒与原型汉滩病毒76 - 118株以及来自前南斯拉夫的类似汉滩病毒分离株福伊尼察和普利特维采之间存在广泛的交叉反应。通过使用一组单克隆抗体进行间接免疫荧光测定，该分离株的反应模式与原型汉滩病毒相似。此外，通过用五种限制性内切酶分析该病毒的聚合酶链反应产物，证实了分离株的特异性。这似乎是欧洲首次从HFRS死亡病例中分离出原型汉滩病毒株。
