Sinchaikul Supachok, Sookkheo Boonyaras, Topanuruk Supachai, Juan Hsueh-Fen, Phutrakul Suree, Chen Shui-Tein
Institute of Biological Chemistry, Academia Sinica, 128 Yen Chiu Yuan Rd., Sec II, Nankang, Taipei 11529, Taiwan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 May 5;771(1-2):261-87. doi: 10.1016/s1570-0232(02)00054-5.
The ability of bioinformatics to characterize genomic and proteomic sequences from bacteria Bacillus sp. for prediction of genes and proteins has been evaluated. Genomics coupling with proteomics, which is relied on integration of the significant advances recently achieved in two-dimensional (2-D) electrophoretic separation of proteins and mass spectrometry (MS), are now important and high throughput techniques for qualifying and analyzing gene and protein expression, discovering new gene or protein products, and understanding of gene and protein functions including post-genomic study. In addition, the bioinformatics of Bacillus sp. is embraced into many databases that will facilitate to rapidly search the information of Bacillus sp. in both genomics and proteomics. It is also possible to highlight sites for post-translational modifications based on the specific protein sequence motifs that play important roles in the structure, activity and compartmentalization of proteins. Moreover, the secreted proteins from Bacillus sp. are interesting and widely used in many applications especially biomedical applications that are the highly advantages for their potential therapeutic values.
