Seo Jong Bok, Kim Hye Sook, Jung Gyoo Yeol, Nam Myung Hee, Chung Joo Hee, Kim Jin Young, Yoo Jong Shin, Kim Chan Wha, Kwon Ohoak
Seoul Branch, Korea Basic Science Institute, Seoul.
Proteomics. 2004 Nov;4(11):3654-9. doi: 10.1002/pmic.200401025.
Psychrophilicity of Gram-positive bacterium, Bacillus psychrosaccharolyticus was investigated in a proteomic approach. One hundred and thirty-one protein spots were analyzed by electrospray ionization-quadrupole-time of flight-tandem mass spectrometry and identified using an unpublished translated contig database as well as a nonredundant Gram-positive bacteria protein database from NCBI because of the lack of a genome sequence of this organism. Results focused on proteomic behavior of cold-response show that global up-regulation of metabolic functions and protective mechanism by stress responses might play a major role in psychrophilicity of B. psychrosaccharolyticus.
采用蛋白质组学方法对革兰氏阳性嗜冷菌解糖嗜冷芽孢杆菌的嗜冷性进行了研究。由于该菌株缺乏基因组序列，通过电喷雾电离-四极杆-飞行时间串联质谱对131个蛋白点进行了分析，并使用未发表的翻译重叠群数据库以及NCBI的非冗余革兰氏阳性菌蛋白质数据库进行了鉴定。聚焦于冷响应蛋白质组行为的结果表明，应激反应引起的代谢功能和保护机制的整体上调可能在解糖嗜冷芽孢杆菌的嗜冷性中起主要作用。
