[日本血吸虫：用日本血吸虫谷胱甘肽 S-转移酶-日本血吸虫 32 免疫小鼠诱导的保护性免疫]

[Schistosoma japonicum: protective immunity induced in mice immunized with SjGST-Sj32].

作者信息

Cai C, Yi X, Zeng X, Zhou J, Shu X

机构信息

Department of Parasitology, Hunan Medical University, Changsha 410078.

出版信息

Hunan Yi Ke Da Xue Xue Bao. 1999;24(3):225-8.

PMID:12016789

Abstract

Schistosoma japonicum glutathione-s-transferase (SjGST), Schistosoma japonicum 32,000 protein (Sj32) and SjGST - Sj32 were expressed and purified to study on protective immunity against Schistosoma japonicum. The result showed that compared with the adjuvant control group, the worm burden was decreased significantly in mice immunized with recombinant SjGST - Sj32, Sj32 and Sj32 + SjGST (P < 0.05). At the same time, the liver egg per gram(EPG) was decreased in mice immunized with SjGST - Sj32, Sj32, Sj32 + SjGST and SjGST. It is concluded that Sj32 significantly induces both protective immunity and anti-fecundity immunity, but SjGST only has an anti-fecundity effect.

摘要

日本血吸虫谷胱甘肽 - S - 转移酶（SjGST）、日本血吸虫32000蛋白（Sj32）以及SjGST - Sj32被表达并纯化，以研究针对日本血吸虫的保护性免疫。结果显示，与佐剂对照组相比，用重组SjGST - Sj32、Sj32以及Sj32 + SjGST免疫的小鼠虫负荷显著降低（P < 0.05）。同时，用SjGST - Sj32、Sj32、Sj32 + SjGST以及SjGST免疫的小鼠每克肝脏虫卵数（EPG）降低。得出的结论是，Sj32显著诱导保护性免疫和抗生殖力免疫，但SjGST仅具有抗生殖力作用。