Hong Z, Wu Y, Wu M, Zhuo X
College of Parmacy, Second Military Medical University, Shanghai 200433.
Yao Xue Xue Bao. 1998 Aug;33(8):616-20.
This paper presents a method to detect the main metabolites of heroin: 6-monoacetylmorphine (6-MAM) and morphine (MOR) in human hair using GC/MS-SIM. The hair specimens were washed with special solvents and cut into about 0.5 mm pieces. Ethylmorphine was added as internal standard and HCl solution for hydrolysis. After hydrolysis, 6-MAM and MOR were extracted by a mixture of solvents (chloroform-isopropyl alcohol-heptane 50:17:33). The residue of the extract was derivatized with N-methyl-N-trimethylsilyl trifluoroacetamide(MSTFA), then the trimethylsilyl(TMS)-derivatives were qualitatively and quantitatively analyzed using GC/MS-SIM. The correlation coefficients for 6-MAM and MOR were 0.9996 and 0.9997, respectively. The recoveries of both 6-MAM and MOR were over 50%. The RSD of within-day and between-day was less than 8% and 10%, respectively. The lower limit of detection of both 6-MAM and MOR was 0.5 ng.mg-1. Hair samples of 12 drug abusers were analyzed using this method, 8 of them gave positive results. This method is simple, accurate and sensitive. It is very suitable for routine case work.
本文介绍了一种使用气相色谱/质谱选择离子监测（GC/MS-SIM）技术检测人发中 heroin 主要代谢物：6-单乙酰吗啡（6-MAM）和吗啡（MOR）的方法。毛发样本用特殊溶剂清洗后切成约 0.5 毫米的片段。加入乙基吗啡作为内标，并加入盐酸溶液进行水解。水解后，用混合溶剂（氯仿-异丙醇-庚烷 50:17:33）萃取 6-MAM 和 MOR。萃取物的残渣用 N-甲基-N-三甲基硅烷基三氟乙酰胺（MSTFA）进行衍生化，然后使用 GC/MS-SIM 对三甲基硅烷基（TMS）衍生物进行定性和定量分析。6-MAM 和 MOR 的相关系数分别为 0.9996 和 0.9997。6-MAM 和 MOR 的回收率均超过 50%。日内和日间相对标准偏差分别小于 8%和 10%。6-MAM 和 MOR 的检测下限均为 0.5 ng·mg-1。使用该方法对 12 名吸毒者的毛发样本进行分析，其中 8 人结果呈阳性。该方法简单、准确、灵敏，非常适合常规案件工作。
