Qiu J, Zhang X Q, Lu Y, Ding M X
College of Life Sciences, Peking University, Beijing 100871.
Shi Yan Sheng Wu Xue Bao. 1998 Sep;31(3):251-8.
In our experiments, protein synthesis of host cells were inhibited quickly at the early stage of infection by Sindbis virus. Polysome and mRNA of host cell fell off from cytoskeletons, whereas virus RNA bound up. We also found it was via 3'-terminal that virus RNA bound with cytoskeleton. After studying on the virus nonstructural proteins, we found the synthesis and processing of virus protein in vitro were far slowly than in vivo, and most of proteins were premature. So, the cytoskeletons may play an important role there. After treated with colchicine and cytochalasin B, the microtubule and microfilament were destroyed. However, the synthesis and processing of nonstructural proteins of Sindbis virus didn't change much, while the structural proteins were inhibited largely. These results showed the differences of dependence of the synthesis of the two kinds of proteins on cytoskeletons. Microtubule and microfilament may be more important to the synthesis of structural proteins than to that of the nonstructural proteins.
在我们的实验中，辛德毕斯病毒感染宿主细胞的早期，宿主细胞的蛋白质合成迅速受到抑制。宿主细胞的多核糖体和信使核糖核酸从细胞骨架上脱落，而病毒核糖核酸则与之结合。我们还发现病毒核糖核酸是通过3'-末端与细胞骨架结合的。在对病毒非结构蛋白进行研究后，我们发现病毒蛋白在体外的合成和加工远比在体内缓慢，并且大多数蛋白都是不成熟的。因此，细胞骨架在其中可能起着重要作用。用秋水仙碱和细胞松弛素B处理后，微管和微丝被破坏。然而，辛德毕斯病毒非结构蛋白的合成和加工变化不大，而结构蛋白则受到很大抑制。这些结果显示了这两种蛋白合成对细胞骨架依赖性的差异。微管和微丝对结构蛋白合成的重要性可能高于对非结构蛋白合成的重要性。
