Ma J, Xie J, Sun W, Jia Z
Department of Pharmacy, General Hospital of Lanzhou, Command of PLA, Lanzhou 730050.
Yao Xue Xue Bao. 1998 May;33(5):369-72.
The restricted access stationary phase (RAS) are a new type of chromatographic packing material having a hydrophobic interior covered with a hydrophilic barrier. The RAS is used for direct injection in high-performance liquid chromatography for determination of drugs in biological fluids. These stationary phases include internal surface reverse phase, semipermeable surface, shielded hydrophobic phase and mixed functional phase (MFP). A kind of MFP packing material was synthesized from a porous silica gel-introduction of hydrophobic phenyl phase and introduction of hydrophilic diol phase. The MFP of RAS, on which proteins were eluted in the void volume, while the drugs were retained and separated, can be used for direct injection analysis of hydrophilic and hydrophobic drugs in serum and plasma. The recovery of the drugs in human serum was almost 100% with good reproducibility.
受限进样固定相（RAS）是一种新型的色谱填充材料，其内部为疏水结构，表面覆盖有亲水屏障。RAS用于高效液相色谱中的直接进样，以测定生物流体中的药物。这些固定相包括内表面反相、半透表面、屏蔽疏水相和混合功能相（MFP）。一种MFP填充材料是由多孔硅胶合成的，引入了疏水苯基相和亲水二醇相。RAS的MFP可用于血清和血浆中亲水和疏水药物的直接进样分析，在该固定相上蛋白质在空体积中被洗脱，而药物被保留和分离。人血清中药物的回收率几乎为100%，重现性良好。
