Nimura N, Itoh H, Kinoshita T
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
J Chromatogr A. 1995 Jan 13;689(2):203-10. doi: 10.1016/0021-9673(94)00896-h.
Direct serum injection for drug determinations can be achieved on a diol-bonded silica gel as a restricted access packing. The diol-bonded phase, 3-(2,3-dihydroxypropoxy)propylsilylsilica, contains two different functions, a hydrophilic function at the tip of the single chemical bond and a hydrophobic function on the inside part of the bond to form a "binary-layered phase" on the support surface. Proteins, as large molecules, contact only the hydrophilic surface of the diol phase, and they are eluted at the solvent front based on size-exclusion chromatography. On the other hand, small molecules such as synthetic drugs are retained on the internal hydrophobic function and separate based on reversed-phase chromatography. Accordingly, the diol-bonded silica gel performs as a restricted access packing for direct serum injection for the determination of relatively hydrophobic drugs.
用于药物测定的直接血清注射可在二醇键合硅胶作为限进填料上实现。二醇键合相，即3-(2,3-二羟基丙氧基)丙基硅烷基硅胶，包含两种不同的功能，在单一化学键末端的亲水性功能以及在键内部的疏水性功能，从而在载体表面形成“二元层相”。蛋白质作为大分子，仅接触二醇相的亲水性表面，并基于尺寸排阻色谱在溶剂前沿被洗脱。另一方面，诸如合成药物之类的小分子保留在内部疏水性功能上，并基于反相色谱进行分离。因此，二醇键合硅胶可作为限进填料用于直接血清注射以测定相对疏水的药物。
