Chowdhury Parimal, Soulsby Michael
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Ann Clin Lab Sci. 2002 Spring;32(2):188-92.
This study tested whether or not simulated weightlessness by tail-suspension increases the levels of lipid peroxidation products in rat brain. The brain tissues of rats on a soybean diet were also assayed for lipid peroxidation products to evaluate the possible role of soy-protein as a dietary anti-oxidant. Male Sprague-Dawley rats were used. Group 1 rats were fed standard Purina rat chow ad libidum and served as controls. Group 2 rats were fed a soybean diet containing 37% soy-protein and were not tail-suspended. Group 3 rats were fed standard Purina rat chow ad libidum and were tail-suspended to induce simulated weightlessness. After 2 wk, all of the rats were killed. Each whole brain was segmented into frontal cortex, cerebellum, and brain stem. After a specific weight of each segment was excised, the residual tissues were combined and used as a whole brain sample. The samples were analyzed for lipid peroxidation products by a chromogenic assay that reacts with malondialdehyde (MDA) and 4-hydroxyalkenals (4-HNE). The mean concentrations of lipid peroxidation products (MDA plus 4-HNE) in whole brain, frontal cortex, cerebellum, and brain stem of the control rats ranged from 16 to 18 micromol/g; the corresponding means ranged from 10 to 13 micromol/g in rats fed the soybean diet, and from 22 to 26 micromol/g in the tail-suspended rats. Thus, the mean levels of lipid peroxidation products in brain tissues were decreased in the rats fed the soy diet and were increased in the rats that were tail-suspended to simulate weightlessness, when compared to those of rats fed a regular diet.
本研究检测了通过尾部悬吊模拟失重是否会增加大鼠脑内脂质过氧化产物的水平。还对食用大豆饮食的大鼠脑组织进行了脂质过氧化产物检测，以评估大豆蛋白作为膳食抗氧化剂的可能作用。使用雄性斯普拉格-道利大鼠。第1组大鼠随意喂食标准普瑞纳大鼠饲料，作为对照组。第2组大鼠喂食含37%大豆蛋白的大豆饮食，且未进行尾部悬吊。第3组大鼠随意喂食标准普瑞纳大鼠饲料，并进行尾部悬吊以诱导模拟失重。2周后，所有大鼠均被处死。将每个完整大脑分为额叶皮质、小脑和脑干。切除每个部分特定重量后，将剩余组织合并作为全脑样本。通过与丙二醛（MDA）和4-羟基烯醛（4-HNE）反应的显色测定法分析样本中的脂质过氧化产物。对照组大鼠全脑、额叶皮质、小脑和脑干中脂质过氧化产物（MDA加4-HNE）的平均浓度范围为16至18微摩尔/克；喂食大豆饮食的大鼠相应平均值范围为10至13微摩尔/克，尾部悬吊的大鼠为22至26微摩尔/克。因此，与喂食常规饮食的大鼠相比，喂食大豆饮食的大鼠脑组织中脂质过氧化产物的平均水平降低，而尾部悬吊模拟失重的大鼠则升高。
